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Status: Completed.
A newly discovered type of immune cells termed Group 2 Innate Lymphoid Cells (ILC2s) promote repair of structural cells after injury in multiple sites. The role of these cells in the kidneys is poorly understood; we believe these cells are pivotal in repair after injury to the kidneys, and that these cells may prevent the development of chronic kidney disease. Through increasing our knowledge of these cells, we hope to one day identify new therapeutic targets.
This proposal is an expansion of the project I successfully completed between 2015-2016 entitled Transcriptome analysis of developing mouse lung following early life infection. In the previous project I analysed large microarray datasets from our own experiments and contrasted them against independently generated data; using high throughput gene analysis tools I applied sophisticated bioinformatics algorithms to this gene expression data. The results from this project were used to formulate the hypothesis for my own 3rd year project, and also helped form a basis for investigating kidneys. Following this project, I assisted with producing the preliminary data for the DECRA proposal and aided with experiments his current PhD candidates were undertaking.
With this project I’ve utilised cutting edge technologies including flow cytometry and 3D imaging in addition to techniques used for analysis of kidney function. The outputs from this project will not only serve as a foundation for my upcoming honours project, but will also provide preliminary data for my supervisors upcoming grant applications. Furthermore, I have been heavily involved in other work involving qPCR and analysing lung function parameters of transgenic mice.
My future direction will be to further my knowledge though honours and PhD projects and attempt to fill gaps in the literature regarding the regulation of this critical cell type. Following graduation, I’d like to continue my research and aim for a research fellowship, whilst still being involved with the university as a lecturer, lab demonstrator and course coordinator. Being in a position to inspire the next generation of scientists is ultimately where I’m aiming to be.
This proposal is an expansion of the project I successfully completed between 2015-2016 entitled Transcriptome analysis of developing mouse lung following early life infection. In the previous project I analysed large microarray datasets from our own experiments and contrasted them against independently generated data; using high throughput gene analysis tools I applied sophisticated bioinformatics algorithms to this gene expression data. The results from this project were used to formulate the hypothesis for my own 3rd year project, and also helped form a basis for investigating kidneys. Following this project, I assisted with producing the preliminary data for the DECRA proposal and aided with experiments his current PhD candidates were undertaking.
With this project I’ve utilised cutting edge technologies including flow cytometry and 3D imaging in addition to techniques used for analysis of kidney function. The outputs from this project will not only serve as a foundation for my upcoming honours project, but will also provide preliminary data for my supervisors upcoming grant applications. Furthermore, I have been heavily involved in other work involving qPCR and analysing lung function parameters of transgenic mice.
My future direction will be to further my knowledge though honours and PhD projects and attempt to fill gaps in the literature regarding the regulation of this critical cell type. Following graduation, I’d like to continue my research and aim for a research fellowship, whilst still being involved with the university as a lecturer, lab demonstrator and course coordinator. Being in a position to inspire the next generation of scientists is ultimately where I’m aiming to be.
